One β Hairpin Follows the Other: Exploring Refolding Pathways and Kinetics of the Transmembrane b-Barrel Protein OmpG

1. Mehdi Damaghi, 
2. Stefan Köster, 
3. Christian A. Bippes, 
4. Özkan Yildiz, and 
5. Daniel J. Müller

1. One by one: The β-barrel-forming outer-membrane protein G (OmpG) from E. coli
2.  can be folded into the native lipid membrane by using single-molecule force spectroscopy. Surprisingly, single β strands do not refold individually but as β hairpins that refold consecutively until the entire β-barrel membrane protein is refolded (see picture). This mechanism significantly advances the understanding of current folding models of β-barrel proteins.

DOI

Journal: Angewandte Chemie International Edition